In the realm of clinical and translational research, the need for reproducible and reliable immune profiling is paramount, especially when samples are handled by different technicians or at various study sites. The Maxpar Direct Immune Profiling System stands out for its proven ability to deliver consistent results run-to-run and site-to-site. This innovative approach utilizes metal tags that are not only more stable than traditional fluorescent tags but also withstand harsh staining protocols and storage conditions, ensuring sample integrity throughout the process. The system’s validated, dry-format assay allows for the identification of major circulating immune cell types in human whole blood or PBMC samples with ease, making it a valuable tool for clinicians seeking routine and reproducible immune response data. With a simple single-tube workflow and automated analysis, variability is minimized, and high reproducibility of multiparameter data is guaranteed. Furthermore, the Maxpar Direct Immune Profiling System offers a solution for maintaining sample integrity between collection and processing by utilizing preservation reagents that stabilize whole blood, even at room temperature. Extensive validation testing has confirmed the system’s high reproducibility across multiple sites, repeatability across sample types, and robust intermediate precision when utilized by different technicians. To streamline data analysis and reporting, the Maxpar Pathsetter software comes into play, automatically identifying 37 immune cell types in FCS files from samples processed with the Maxpar Direct Immune Profiling Assay. This comprehensive guide to immune profiling showcases the power and reliability of the Maxpar system, making it an indispensable tool in the field of immunophenotyping.
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