Sortases play a crucial role in anchoring surface proteins in Gram-positive bacteria, serving as potential targets for novel antibiotics and tools for protein engineering. While Class A sortases are well-studied for recognizing LPXTG motifs, Class B sortases, like Bacillus anthracis SrtB, target distinct sequences such as NPXTG or NPQTN. In this study, through biochemistry, structural […]
Proteins are frequently modified to improve their affinity for ligands, a crucial aspect for therapeutic purposes. Traditionally, methods like phage or yeast display libraries have been used to evolve antibodies and T cell receptors (TCRs) for enhanced affinity. However, these methods have limitations in assessing the entire protein-protein interface rapidly. Combining directed evolution with deep […]
In the realm of synthetic biology, ground-breaking research often hinges on the exploration of nature’s smallest elements, unlocking secrets that have far-reaching implications. The protein titin, a goliath amongst its peers in the animal kingdom and named after the titans of Greek mythology, is the subject of a recent, compelling study by a team at […]
