The HPLC 2025 conference, held in Bruges, Belgium, from June 19 to 25, served as a major event in the chromatography calendar. Spearheaded by an organizing committee that included Gert Desmet, Deirdre Cabooter, Sebaastian Eeltink, and Ken Broeckhoven, the conference reinforced the significance of in-person interactions within the chromatography community. As separation science evolves, driven by technological advancements, sustainable practices, and the integration of artificial intelligence (AI) and machine learning (ML), the need for real-time discussions becomes increasingly evident. This review encapsulates key highlights from the event, showcasing the innovative strides being made in analytical chemistry.
Modern Method Development
The session on modern method development highlighted the pivotal role of data-driven approaches and AI in analytical chemistry. Traditional method development can be complex, costly, and time-consuming, particularly in two-dimensional liquid chromatography (LC×LC). Peter Schoenmakers from the University of Amsterdam emphasized the necessity of computer-assisted tools to manage the intricate interdependencies of method parameters, while still valuing expert knowledge.
Kevin Schug from the University of Texas at Arlington presented a compelling case for machine learning and surrogate optimization in analytical method development. His work with supercritical fluid extraction–supercritical fluid chromatography (SFE–SFC) demonstrated that leveraging structure-based molecular feature encoding can enhance predictive performance and minimize experimental workloads.
The discussion further delved into challenges within ex vivo tissue metabolomics. Gunda Koellensperger from the University of Vienna underscored the importance of strategic sampling and control over analytical variables. Advances in high-resolution mass spectrometry (HRMS) and miniaturized workflows are paving the way for more reliable clinical metabolomics applications.
Debby Mangelings from Vrije Universiteit Brussel introduced quantitative structure enantioselective retention relationship (QSERR) models, which predict chiral separations on polysaccharide-based phases. This innovative approach successfully combines achiral and chiral molecular descriptors, enabling accurate predictions of enantiomer retention and selectivity across a range of pharmaceutical compounds.
Fanyi Duanmu from University College London showcased an AI-driven “Smart HPLC Robot” designed to automate method development. By integrating mechanistic models and machine learning, this system significantly reduces both the time and resources typically required for method development.
Lastly, José-Ramón Torres-Lapasió from the University of Valencia highlighted the efficacy of global retention models in predicting retention shifts in serially coupled columns, particularly under gradient conditions. This insight supports the refinement of hybrid column HPLC strategies, enhancing method reliability.
Pharmaceutical Separation Science Session
The second day of HPLC 2025 culminated in a session dedicated to pharmaceutical separations, led by Debby Mangelings and Todd Maloney from Eli Lilly. Erik Regalado from Merck & Co. initiated the discussions with a presentation on a groundbreaking automated multicolumn ultrahigh-pressure liquid chromatography–hydrophilic interaction chromatography (UHPLC–HILIC) screening workflow. This innovative approach employs multiple UHPLC-compatible columns across diverse pH ranges and solvents, simplifying the HILIC optimization process and promoting high-throughput, standardized method development.
Filip Cuyckens from Johnson & Johnson shared insights on enhancing sensitivity in drug metabolism studies. By focusing on optimized liquid chromatography configurations, he demonstrated how custom LC setups, including online preconcentration and large injection volumes, can significantly improve analysis in complex biological samples.
Gwenael Nys from Sanofi presented a mass spectrometry-based multi-attribute monitoring (MAM) method for quality control testing of therapeutic nanobodies. This technique utilizes LC–MS to monitor multiple product quality attributes simultaneously, offering deeper insights compared to traditional HPLC–UV methods.
Nikoline Juul Nielsen from the University of Copenhagen/Novo Nordisk concluded the session with a presentation on microsampling combined with microflow LC–MS/MS for studying insulin pharmacokinetics in rats. This innovative approach not only enhanced sensitivity but also improved data quality and minimized animal usage, demonstrating a commitment to ethical research practices.
Video Coverage and Editorial Insights
Alasdair Matheson, executive editor of LCGC International, conducted on-site interviews with leading figures in separation science. These discussions provided valuable perspectives on key topics, including sustainability in pharmaceutical practices, as articulated by Paul Ferguson. Additionally, Torgny Fornstedt offered insights into the challenges of oligonucleotide analysis, while Gesa Schad discussed practical applications of supercritical fluid chromatography (SFC).
The event also featured essential editorial content, including Dave Bell’s annual “Column Watch” guide, which highlighted significant trends and technological advances from the conference. The HPLC Companion 2025 further illuminated critical topics such as automation advancements, non-targeted screening workflows, and the evolution of two-dimensional liquid chromatography.
Conclusion
HPLC 2025 served as a platform for showcasing the forefront of innovations in chromatography. The integration of AI, machine learning, and advanced methodologies is reshaping the landscape of analytical chemistry. As the community continues to engage in these transformative discussions, the future of separation science looks promising and dynamic.
- Data-driven approaches enhance method development efficiency.
- AI and automation are crucial for modern analytical workflows.
- Multicolumn strategies streamline pharmaceutical separations.
- Ethical research practices are evolving through innovative methodologies.
- The conference provided a rich exchange of ideas and collaboration opportunities.
Source: www.chromatographyonline.com