Tag: ptaAckAKnockout

CRISPR Knock-Out of pta-ackA & Controlled Fed-Batch Glucose Pulses

1 A Practical Playbook for Killing Acetate in High-Density E. coli Heme Fermentation Target readers: upstream engineers and strain developers who have muttered the question,“How do I suppress acetate overflow in high-density E. coli heme fermentation above 60 g L⁻¹ CDW?” Why acetate becomes public enemy #1 once biomass tops 60 g L⁻¹ Overflow (or […]