1 (Links lead to open-access definitions or primary papers so readers can dive deeper if they like.) How do I keep ΔtrpD Corynebacterium from excreting indole during recombinant tryptophan aldehyde synthesis? Why the purple halo spells real money Plate your engineered ΔtrpD Corynebacterium glutamicum on agar sprayed with Kovács reagent and you may see a […]
Category: Metabolic Engineering / Synthetic Biology
CRISPR Knock-Out of pta-ackA & Controlled Fed-Batch Glucose Pulses
1 A Practical Playbook for Killing Acetate in High-Density E. coli Heme Fermentation Target readers: upstream engineers and strain developers who have muttered the question,“How do I suppress acetate overflow in high-density E. coli heme fermentation above 60 g L⁻¹ CDW?” Why acetate becomes public enemy #1 once biomass tops 60 g L⁻¹ Overflow (or […]
